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The Vibrio harveyi bioassay used routinely to detect AI-2 quorum sensing inhibition is confounded by inconsistent normalization across marine matrices

Author(s): Blair, W.M.; G.J. Doucette


Publication Type: Journal Article

Journal Title: Journal of Microbiological Methods

Date of Publication: 2013

Reference Information: 92(3): 250-252

Keywords: AI-2; bioassay; quorum sensing; quorum quenching; inhibition; Vibrio harveyi

Abstract: The Vibrio harveyi autoinducer-2 (AI-2) bioassay is used routinely to screen for inhibition of the AI-2 quorum sensing system. The present study utilizes three well-described bacterial strains to demonstrate that inconsistent normalization across matrices undermines the assay’s use in screening marine samples for AI-2 inhibition.